Describe the Procedure for Making a Smear Using Sterile Technique

The sterile field is used in many situations outside the operating room. The purpose of creating a sterile field is to reduce the number of microbes present to as few as possible.

Worksheet No 2 Aseptic Technique And Bacterial Smear Preparation Medical Technology Studocu

Identify the parameters of a sterile field.

. A simple stain is the use of one dye. Using a sterile pipette add one drop of sterile saline or sterile water to the center of the microscope slide. Air dry and heat fix the organism on a glass slide and cover with a square of blotting paper or toweling cut.

Allow all slides to. Plate cover is taken off and held diagonally over. BASIC STEPS IN STERILE TECHNIQUE.

Minimize the amount of agar and the length of time the agar is exposed to the environment during the streak process. Describe practices that reduce the spread of infection when preparing or working in a sterile environment. Next using a sterile loop or straight wire needle transfer a bit of the growth to the drop of water and rub the needle around until the.

1 avoid sudden movements of body parts covered by sterile drapes. Loops are used to inoculate or streak petri plates. Three Sector Streak t streak.

2refrain from touching sterile supplies drapes or nurses gloves and gown. Sterilize the wire loop. Ask a non-sterile person to step aside rather than trying to crowd past him.

Avoid dig into the medium or scrape on it. Turn back to a non-sterile person or when passing. Using a loop place one to two loops of water on center of slide.

With a sterile loop touch the entire loop to the culture and emulsify the cells in water on Slide. The delivery of sterile products for use in patient care depends not only on the effectiveness of the sterilization process but also on the unit design decontamination disassembling and. Sterile technique is a set of specific practices and procedures performed to make equipment.

Use the special flap to pull back the paper wrapper of your pad or kit. Take a clean grease free slide and make smear using sterile technique. 3 avoid coughing sneezing or talking over.

Mix this properly by. Care should be given not to. Culture from solid media.

In sterile technique only sterile surfaces touch other sterile surfaces and exposure to the air is kept to a minimum. Open it so that the inside faces away from you. Place the culture you wish to.

An agar plate is taken and labeled properly. Use the malachite green primary stain stain solution. It requires a sterile room gloves gowns caps tools and masks along.

Sterile technique is used in surgeries and other large invasive procedures where infection could be the most dangerous. Face a sterile area when passing the area. Pinch the other sections on the outside and pull them.

In order to heat fix a bacterial smear it is necessary to first let the bacterial sample air dry. Gently drop items onto the sterile field or use sterile forceps to place sterile items onto the field. Wash hands thoroughly before beginning any sterile procedure.

Once a bacterial sample is heat fixed to a microscope slide there are many staining techniques that can be used to view the bacteria. General procedure of aseptic technique petri plate inoculations -petri plate inoculations. Surgical asepsis is the absence of all microorganisms within any type of invasive procedure.

Place a small drop of water on a clean grease-free slide. Then either place the slide in the slide holder of a microincinerator or pass the dried slide through. Make smear by sterile technique using clean grease.

Set up for assist with and perform sterile procedures in a clean environment. To make a bacterial plate culture use a cooled sterile loop and place a. Air dry and heat fix the organism on a glass slide and cover with a blotting paper.

Light the Bunsen burner. Using a sterile flamed loop a loopful sample is carefully spread in an edge of the agar. Aseptically pick a small amount of an.

A suitable smear allows examination of isolated cells and their characteristic cellular arrangement. If using equipment wrapped in linen ensure sterility by checking the tape for date and to view. Take a small portion of bacterial cell on the tip of the loop and transfer it into the droplet of sterile distilled water.

Antt Pha Infection Control

Aseptic Technique Uses Benefits And Complications

2

Microbiology Lab Quiz 2 Flashcards Quizlet